Description
Aviva Systems Biology Aspergillus fumigatus IgM ELISA Kit (Human) (OKNX00104) is based on standard reverse capture sandwich enzyme-linked immuno-sorbent assay technology. Aspergillus fumigatus antigen has been pre-coated and blocked in a 96-wellplate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and washed. An HRP conjugated detector antibody specific for Human IgM is added, incubated and followed by washing. An enzymatic reaction is produced through the addition of substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration is read by absorbance at 450 nm and is qualitatively proportional to the amount of sample anti-Aspergillus fumigatus IgM captured the in well